Purified PTP1 protein induces antigen-specific protective immunity against Encephalitozoon cuniculi

Humoral intestinal immunity against Encephalitozoon cuniculi (Microsporidia) infection in mice.

Three strains of mice, BALB/c, IL-12 knock-out (KO) and INF-gamma knock-out, were chosen as an experimental model for the study of intestinal immunity induction against Encephalitozoon cuniculi Levaditi, Nicolau et Schoen, 1923 infection. Mice were infected perorally with 10(7) spores and re-infected with the same dose 70 days after the first infection. The anti-E. cuniculi IgA, IgG and IgM res...

Immune Response Against Encephalitozoon cuniculi Infection

Encephalitozoon cuniculi Infection in Rabbits

intracellular parasite in the phylum Microspora, which is defined by its resistant spore stage. Microsporidia receive little attention in veterinary medical curricula because they are considered important only as parasites of rabbits and fish. Microsporidia are important parasites of humans as well, with some species causing severe disease and death in immunocompromised individuals. Microsporid...

Monoclonal antibodies for specific detection of Encephalitozoon cuniculi.

Seven species-specific monoclonal antibodies (MAbs) were produced against Encephalitozoon cuniculi and characterized. The MAbs were immunoglobulin G, and when used for indirect microimmunofluorescence microscopy and Western immunoblot assays, they detected E. cuniculi originating from clinical samples. They did not cross-react with other Encephalitozoon species (E. intestinalis and E. hellem) o...

Identification of a new spore wall protein from Encephalitozoon cuniculi.

Microsporidia form environmentally resistant spores that are critical for their host-to-host transmission and persistence in the environment. The spore walls of these organisms are composed of two layers, the exospore and the endospore. Two spore wall proteins (SWP1 and SWP2) have been previously identified in members of the Encephalitozoonidae family. These proteins localize to the exospore. T...